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Hu, A 
ORCID: https://orcid.org/0000-0001-8821-6591,
2024.
The investigation of Elf5 during skin development and homeostasis.
PhD, Nottingham Trent University.
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Abstract
In mammals, skin as a complex organ necessitates exact control over the growth and differentiation in both spatial and temporal aspects, essential for the organism's critical functions. Skin development and homeostasis are complex dynamic processes that include the formation and maintaining of the homeostasis of the epidermis, a stratified self-renewing epithelium and its appendices, such as hair follicles. Elf5 is a member of the epithelium-specific Ets subfamily of transcription factors and is essential for epithelial development, homeostasis and prevention of tumorigenesis. However, the role of Elf5 in regulating skin and hair follicle (HF) development/homeostasis and regeneration remains largely unknown. This study brings new insights into novel expression patterns of Elf5 correlating with functionally important regulation of cellular processes involving proliferation and differentiation in stem/progenitor cell populations in skin and HFs. Utilising colony forming assay with FACS-sorted mouse basal and HF-bulge stems cells (SCs), I observed reduced ability of these SCs to form colonies after gain and/or loss of Elf5 functions. Target identification of Elf5-associated pathways was conducted using RNA and ChIP sequencing analysis in both primary keratinocytes and SCs of skin and HFs. This identified that cell proliferation and differentiation are potential functions regulated by Elf5. I hypothesise that Elf5 plays a crucial role in controlling skin-SCs self-renewal, proliferation and differentiation and/or their balance during skin and hair development/homeostasis and regeneration. This study offers novel insight into the biological importance of Elf5 during skin and hair development, homeostasis and the possible use of Elf5 and its targets for potential stem cell-based therapies for skin and hair disorders in the near future.
| Item Type: | Thesis |
|---|---|
| Creators: | Hu, A. |
| Contributors: | Name Role NTU ID ORCID |
| Date: | January 2024 |
| Rights: | This work is the intellectual property of the author. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed in the owner(s) of the Intellectual Property Rights. |
| Divisions: | Schools > School of Science and Technology |
| Record created by: | Melissa Cornwell |
| Date Added: | 28 Jan 2025 15:17 |
| Last Modified: | 28 Jan 2025 15:17 |
| URI: | https://irep.ntu.ac.uk/id/eprint/52936 |
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