Optical scattering methods for the label-free analysis of single biomolecules

Gordon, R, Peters, M and Ying, C ORCID: https://orcid.org/0000-0002-7279-1388, 2024. Optical scattering methods for the label-free analysis of single biomolecules. Quarterly Reviews of Biophysics, 57: e12. ISSN 0033-5835

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Abstract

Single-molecule techniques to analyze proteins and other biomolecules involving labels and tethers have allowed for new understanding of the underlying biophysics; however, the impact of perturbation from the labels and tethers has recently been shown to be significant in several cases. New approaches are emerging to measure single proteins through light scattering without the need for labels and ideally without tethers. Here, the approaches of interference scattering, plasmonic scattering, microcavity sensing, nanoaperture optical tweezing, and variants are described and compared. The application of these approaches to sizing, oligomerization, interactions, conformational dynamics, diffusion, and vibrational mode analysis is described. With early commercial successes, these approaches are poised to have an impact in the field of single-molecule biophysics.

Item Type:	Journal article
Publication Title:	Quarterly Reviews of Biophysics
Creators:	Gordon, R., Peters, M. and Ying, C.
Publisher:	Cambridge University Press
Date:	2024
Volume:	57
ISSN:	0033-5835
Identifiers:	Number Type 10.1017/s0033583524000088 DOI 2283743 Other
Rights:	This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	10 Feb 2025 13:27
Last Modified:	10 Feb 2025 13:27
URI:	https://irep.ntu.ac.uk/id/eprint/53012

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