Distinct molecular profiles underpin mild-to-moderate equine asthma cytological profiles

Karagianni, AE, Richard, EA, Toquet, M-P, Hue, ES, Courouce-Malblanc, A, McGorum, B, Kurian, D, Aguilar, J, Mazeri, S, Wishart, TM ORCID: https://orcid.org/0000-0002-1973-6654 and Pirie, RS, 2024. Distinct molecular profiles underpin mild-to-moderate equine asthma cytological profiles. Cells, 13 (22): 1926. ISSN 2073-4409

Preview

Text 2371166_Wishart.pdf - Published version Download (6MB) | Preview

Abstract

A state-of-the-art multi-omics approach was applied to improve our understanding of the aetio-pathogenesis of a highly prevalent, performance-limiting disorder of racehorses: mild-to-moderate equine asthma (MMEA). This is a prerequisite to improving prophylactic, management, and therapeutic options for this condition. Although a number of risk factors have been identified, options for intervention are limited. This study applied a multi-omic approach to reveal key inflammatory pathways involved in inflammatory cell recruitment to the lower airways and highlight distinct MMEA inflammatory profiles. We compared bronchoalveolar lavage fluid (BALF) cell gene and protein expression data from horses with non-inflammatory BALF cytology with those isolated from horses with neutrophilic, mastocytic, mixed neutrophilic/mastocytic, and eosinophilic/mastocytic inflammation. The analyses on transcriptomic/proteomic data derived from BALF from horses with neutrophilic cytology showed enrichment in classical inflammatory pathways, and horses with mastocytic inflammation showed enrichment in pathways involved in hypersensitivity reactions related to nonclassical inflammation potentially mimicking a Th2-immune response. The mixed eosinophilic/mastocytic group also presented with a nonclassical inflammatory profile, whereas the mixed neutrophilic/mastocytic group revealed profiles consistent with both neutrophilic inflammation and hypersensitivity. Our adopted multi-omics approach provided a holistic assessment of the immunological status of the lower airways associated with the different cytological profiles of equine asthma.

Item Type:	Journal article
Publication Title:	Cells
Creators:	Karagianni, A.E., Richard, E.A., Toquet, M.-P., Hue, E.S., Courouce-Malblanc, A., McGorum, B., Kurian, D., Aguilar, J., Mazeri, S., Wishart, T.M. and Pirie, R.S.
Publisher:	MDPI
Date:	20 November 2024
Volume:	13
Number:	22
ISSN:	2073-4409
Identifiers:	Number Type 10.3390/cells13221926 DOI 2371166 Other
Rights:	© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	19 Feb 2025 13:42
Last Modified:	19 Feb 2025 13:42
URI:	https://irep.ntu.ac.uk/id/eprint/53081

Actions (login required)

Edit View

Statistics

Views

Downloads