Hepatic hydrogen sulfide levels are reduced in mouse model of Hutchinson-Gilford progeria syndrome

Wilkie, SE, Marcu, DE, Carter, RN, Morton, NM ORCID: https://orcid.org/0000-0001-8218-8462, Gonzalo, S and Selman, C, 2023. Hepatic hydrogen sulfide levels are reduced in mouse model of Hutchinson-Gilford progeria syndrome. Aging, 15 (12), pp. 5266-5278. ISSN 1945-4589

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Abstract

Hutchinson-Gilford progeria syndrome (HGPS) is a rare human disease characterised by accelerated biological ageing. Current treatments are limited, and most patients die before 15 years of age. Hydrogen sulfide (H2S) is an important gaseous signalling molecule that it central to multiple cellular homeostasis mechanisms. Dysregulation of tissue H2S levels is thought to contribute to an ageing phenotype in many tissues across animal models. Whether H2S is altered in HGPS is unknown. We investigated hepatic H2S production capacity and transcript, protein and enzymatic activity of proteins that regulate hepatic H2S production and disposal in a mouse model of HGPS (G609G mice, mutated Lmna gene equivalent to a causative mutation in HGPS patients). G609G mice were maintained on either regular chow (RC) or high fat diet (HFD), as HFD has been previously shown to significantly extend lifespan of G609G mice, and compared to wild type (WT) mice maintained on RC. RC fed G609G mice had significantly reduced hepatic H2S production capacity relative to WT mice, with a compensatory elevation in mRNA transcripts associated with several H2S production enzymes, including cystathionine-γ-lyase (CSE). H2S levels and CSE protein were partially rescued in HFD fed G609G mice. As current treatments for patients with HGPS have failed to confer significant improvements to symptoms or longevity, the need for novel therapeutic targets is acute and the regulation of H2S through dietary or pharmacological means may be a promising new avenue for research.

Item Type:	Journal article
Publication Title:	Aging
Creators:	Wilkie, S.E., Marcu, D.E., Carter, R.N., Morton, N.M., Gonzalo, S. and Selman, C.
Publisher:	Impact Journals LLC
Date:	2023
Volume:	15
Number:	12
ISSN:	1945-4589
Identifiers:	Number Type 10.18632/aging.204835 DOI 2465468 Other
Rights:	© 2023 Wilkie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	10 Jul 2025 15:04
Last Modified:	10 Jul 2025 15:04
URI:	https://irep.ntu.ac.uk/id/eprint/53928

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