Anti-inflammatory, antioxidant, and antimicrobial evaluation of morin

Sales, LS, Hewitt, B, Muchova, M, Brighenti, FL, Kuehne, SA ORCID: https://orcid.org/0000-0001-6790-8433, Grant, MM and Milward, MR, 2025. Anti-inflammatory, antioxidant, and antimicrobial evaluation of morin. Archives of Oral Biology, 178: 106343. ISSN 0003-9969

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Abstract

Objectives: This study aimed to evaluate the anti-inflammatory and antioxidant activity of morin in an epithelial cell culture inflammatory model, as well as its antimicrobial activity against periodontal pathogens. Effects of morin alone or slow-release from a polymer-based formulation were compared.
Methods: An oral epithelial cell line (H400) was pre-conditioned with 0.125 mg/mL morin (free morin or morin in the formulation) for 8 h. The cells were challenged with heat-killed Fusobacterium nucleatum and Porphyromonas gingivalis (MOI 100:1) for 24 h. The production of GM-CSF was measured by enzyme-linked immunosorbent assay (ELISA) and the gene expression levels of IL-8, GM-CSF, IL1B, NF-kB and NLRP3 were evaluated by RT – PCR. Neutrophils were pre-conditioned with 0.01 mg/mL morin and stimulated immediately with heat-killed F. nucleatum or P. gingivalis (MOI 100:1) for 2.5 h and then total reactive-oxygen-species (ROS) levels were determined using enhanced chemiluminescence. F. nucleatum and P. gingivalis as well as a multi-species biofilm (Streptococcus oralis, F. nucleatum and P. gingivalis) were exposed to 1 mg/mL morin for 24 h, planktonic culture and for 7 days, multi-species biofilm. After exposure, the microbial viability (CFU/mL) and biofilm biomass (crystal violet assay) were determined. Live/dead staining and confocal laser scanning microscopy were also performed. Data were submitted to one way ANOVA followed by Tukey's post-hoc test (α=0.05).

Results: Morin and the polymer-based formulation containing morin significantly attenuated secretion of GM-CSF, significantly reduced ROS generation and the gene expression levels of the cytokines tested (p < 0.05). Microbial viability and the biofilm biomass significantly reduced (p < 0.05) after treatment with morin and the polymer-based formulation containing morin.

Conclusions: Morin showed an anti-inflammatory and antioxidant effect as well as antimicrobial properties against periodontal pathogens. In addition, polymer-based formulation enhanced these effects.

Item Type:	Journal article
Publication Title:	Archives of Oral Biology
Creators:	Sales, L.S., Hewitt, B., Muchova, M., Brighenti, F.L., Kuehne, S.A., Grant, M.M. and Milward, M.R.
Publisher:	Elsevier
Date:	October 2025
Volume:	178
ISSN:	0003-9969
Identifiers:	Number Type 10.1016/j.archoralbio.2025.106343 DOI 2460152 Other S0003996925001712 Publisher Item Identifier
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	22 Jul 2025 13:35
Last Modified:	22 Jul 2025 13:35
URI:	https://irep.ntu.ac.uk/id/eprint/53987

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