Deletion of rgaS reduces virulence in a mouse model of Clostridioides difficile infection

Bilverstone, TW, Simpson, ME, Hagspiel, N, Petri, WA, Kuehne, SA ORCID: https://orcid.org/0000-0001-6790-8433 and Minton, NP, 2025. Deletion of rgaS reduces virulence in a mouse model of Clostridioides difficile infection. Anaerobe. ISSN 1075-9964

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Abstract

Two-component system signalling is involved in several processes relevant to Clostridioides difficile virulence. We demonstrate that deletion of the orphan histidine kinase rgaS (R20291_0503), reduces detectable toxin in the supernatant of R20291 and impairs virulence in a mouse model of infection. These data suggest that RgaS regulates virulence capabilities in hypervirulent C. difficile.

Item Type:	Journal article
Publication Title:	Anaerobe
Creators:	Bilverstone, T.W., Simpson, M.E., Hagspiel, N., Petri, W.A., Kuehne, S.A. and Minton, N.P.
Publisher:	Elsevier
Date:	9 August 2025
ISSN:	1075-9964
Identifiers:	Number Type 10.1016/j.anaerobe.2025.102988 DOI 2483743 Other
Rights:	©2025. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Borcherds
Date Added:	14 Aug 2025 08:57
Last Modified:	14 Aug 2025 08:57
URI:	https://irep.ntu.ac.uk/id/eprint/54190

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