Platinum‐binding peptides: binding kinetics, affinities, and facet‐specific conformational adaptation

Michaelis, M, Limo, MJ, Kothur, SR and Perry, CC ORCID: https://orcid.org/0000-0003-1517-468X, 2025. Platinum‐binding peptides: binding kinetics, affinities, and facet‐specific conformational adaptation. European Journal of Inorganic Chemistry. ISSN 1434-1948

Preview

Text 2491457_Perry.pdf - Published version Download (1MB) | Preview

Abstract

Platinum-binding peptides (PtBPs) identified via phage display have emerged as powerful molecular tools for the controlled synthesis and functionalization of nanostructured platinum surfaces. However, the molecular determinants governing their surface recognition, binding strength, and structural adaptability remain incompletely understood. Here, a comparative analysis of five PtBPs, three previously reported (TLTTLTN, SSFPQPN, TLHVSSY) and two newly identified by phage display (TGELSQK, LLVTSVT), using quartz crystal microbalance with dissipation monitoring (QCM-D) and synchrotron radiation circular dichroism (SRCD) spectroscopy, is presented. Adsorption kinetics and binding affinities determined by QCM-D reveal sequence-specific differences in association and dissociation rates, which correlate with the viscoelastic properties of the adsorbed layers. SRCD spectra show that all peptides adopt predominantly disordered conformations in solution but exhibit facet-dependent spectral shifts upon adsorption onto platinum nanoparticles, consistent with conformational adaptation at the interface. The combined data highlight the importance of amino acid composition, kinetic binding parameters, and conformational flexibility in governing Pt–PtBP interactions. This integrated approach provides a deeper understanding of peptide–surface recognition and may support the rational design of sequence-defined biomolecules for use in catalysis, surface modification, and biomedical nanotechnology.

Item Type:	Journal article
Publication Title:	European Journal of Inorganic Chemistry
Creators:	Michaelis, M., Limo, M.J., Kothur, S.R. and Perry, C.C.
Publisher:	Wiley
Date:	1 September 2025
ISSN:	1434-1948
Identifiers:	Number Type 10.1002/ejic.202500224 DOI 2491457 Other
Rights:	© 2025 The Author(s). European Journal of Inorganic Chemistry published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Borcherds
Date Added:	02 Sep 2025 08:37
Last Modified:	02 Sep 2025 08:37
URI:	https://irep.ntu.ac.uk/id/eprint/54272

Actions (login required)

Edit View

Statistics

Views

Downloads