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Eyre, J, 2025. Synthesis of metal nanoparticles in a deep eutectic solvent for use in an antimicrobial wound dressing. PhD, Nottingham Trent University.
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Abstract
Antimicrobial resistance has been predicted to cause more deaths than cancer by the year 2050. This project proposes to use Deep Eutectic Solvents (DES’s) to synthesise silver nanoparticles (AgNPs) for use in an antimicrobial wound dressing. DES’s have previously been studied to synthesise AgNPs as a greener alternative to conventional aqueous methods.
The synthesis of AgNPs using the DES (Bet:Gly 1:3),3 and oleylamine (OAm) as a capping agent was initially studied producing spherical AgNPs that were 10±2.4 nm in size, characterised by UV-vis, DLS and TEM. Additional synthesis and extraction methods were then developed to remove the hazardous OAm to synthesise AgNPs in Bet:Gly 1:3 without any additional reagents which were 13±1.94 nm in size with a spherical morphology suitable for an antimicrobial wound dressing.
Novel three-component DES’s, were developed with inherent antimicrobial activity that could also act as a reducing and capping agent for synthesis of AgNPs. A successful synthesis method with Cap:Lau:Bet 4:2:1 DES was developed which resulted in spherical AgNPs with size ranges of 6.36±2.89 nm. The antimicrobial properties of AgNPs in both a Bet:Gly 1:3 and Cap:Lau:Bet 4:2:1 were studied using colony biofilm assays, as well as minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC), for a Gram-positive bacteria (S. aureus) and a Gram-negative bacteria (E. coli). The best candidate for an antimicrobial wound dressing was AgNPs in Cap:Lau:Bet 4:2:1 which had a more potent antimicrobial effect against the biofilms with MIC and MBC concentrations for both bacterium found to be 24.1 µg/mL and 0.75 µg/mL respectively. This work advanced the synthesis of AgNPs in a DES; allowing for a greater control and stability of the AgNPs, as seen by the narrow size ranges produced, as well as demonstrating a more accurate protocol for the determination of the antimicrobial properties of DESs.
| Item Type: | Thesis |
|---|---|
| Creators: | Eyre, J. |
| Contributors: | Name Role NTU ID ORCID |
| Date: | March 2025 |
| Rights: | The copyright in this work is held by the author. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed to the author. |
| Divisions: | Schools > School of Science and Technology |
| Record created by: | Jeremy Silvester |
| Date Added: | 01 Oct 2025 10:03 |
| Last Modified: | 01 Oct 2025 10:03 |
| URI: | https://irep.ntu.ac.uk/id/eprint/54485 |
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