Sex-specific effects of CD248 on metabolism and the adipose tissue lipidome

Patrick, K, Tian, X, Cartwright, D, Heising, S, Glover, MS, Northall, EN, Cazares, L, Hess, S, Baker, D, Church, C, Davies, G, Lavery, G ORCID: https://orcid.org/0000-0001-5794-748X and Naylor, AJ, 2023. Sex-specific effects of CD248 on metabolism and the adipose tissue lipidome. PLOS ONE, 18 (4): e0284012. ISSN 1932-6203

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Abstract

Cd248 has recently been associated with adipose tissue physiology, demonstrated by reduced weight gain in high fat diet-fed mice with genetic deletion of Cd248 relative to controls. Here we set out to determine the metabolic consequences of loss of Cd248. Strikingly, we find these to be sex specific; By subjecting Cd248-/- and Cd248+/+ mice to a high fat diet and indirect calorimetry study, we identified that only male Cd248-/- mice show reduced weight gain compared to littermate control wildtype mice. In addition, male (but not female) mice showed a lower respiratory exchange ratio on both chow and high fat diets, indicating a predisposition to metabolise lipid. Lipidomic studies on specific fat depots found reduced triglyceride and diglyceride deposition in male Cd248-/- mice, and this was supported by reduced expression of lipogenic and adipogenic genes. Finally, metabolomic analysis of isolated, differentiated preadipocytes found alterations in metabolic pathways associated with lipid deposition in cells isolated from male, but not female, Cd248-/- mice. Overall, our results highlight the importance of sex controls in animal studies and point to a role for Cd248 in sex- and depot-specific regulation of lipid metabolism.

Item Type:	Journal article
Publication Title:	PLOS ONE
Creators:	Patrick, K., Tian, X., Cartwright, D., Heising, S., Glover, M.S., Northall, E.N., Cazares, L., Hess, S., Baker, D., Church, C., Davies, G., Lavery, G. and Naylor, A.J.
Publisher:	Public Library of Science (PLoS)
Date:	28 April 2023
Volume:	18
Number:	4
ISSN:	1932-6203
Identifiers:	Number Type 10.1371/journal.pone.0284012 DOI 2554589 Other
Rights:	© 2023 Patrick et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Divisions:	Schools > School of Science and Technology
Record created by:	Jeremy Silvester
Date Added:	09 Jan 2026 11:13
Last Modified:	09 Jan 2026 11:13
URI:	https://irep.ntu.ac.uk/id/eprint/55002

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