Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology

Rashid, T, Jusoh, M, Zakaria, ZY, Yahya, N, Ansar, S, Kiong, TS and Sher, F ORCID: https://orcid.org/0000-0003-2890-5912, 2024. Design and optimisation of lysozyme protein purification process using non-thermal progressive freeze concentration technology. Chemical Engineering and Processing - Process Intensification, 205: 109975. ISSN 0255-2701

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Abstract

In this study, PFC system is investigated to improve the concentration and yield of lysozyme. The research focused on an attempt to thoroughly construct an ice crystallizer with measurable and optimized design parameters for an efficient lysozyme protein concentration procedure because the productivity of PFC is always an issue. A new Multiple Probe Cryo-Concentrator (MPCC) device was designed and successfully equipped with probes having a well-distributed cooled surface area for ice crystallization with proper internal cooling temperature control as well as a solution movement mechanism provided by a stirrer in the tank. The impact of different operating parameters is optimally investigated. Central Composite Design (CCD) is utilized to optimize PFC operating conditions and their response to partition constant (K-value) and solute yield. The results showed that a coolant temperature of -12 ⁰C, stirrer speed of 350 rpm, operation time of 40 min and initial concentration of 10 mg/mL gave the best K-value (0.132) and solute concentration yield (87.39 %). The design elements of the equipment are crucial in providing improved PFC performance. The study revealed that the PFC system designed and applied in this study can improve the lysozyme protein concentration as needed in the food and pharmaceutical industry.

Item Type:	Journal article
Publication Title:	Chemical Engineering and Processing - Process Intensification
Creators:	Rashid, T., Jusoh, M., Zakaria, Z.Y., Yahya, N., Ansar, S., Kiong, T.S. and Sher, F.
Publisher:	Elsevier BV
Date:	November 2024
Volume:	205
ISSN:	0255-2701
Identifiers:	Number Type 10.1016/j.cep.2024.109975 DOI 2556392 Other
Rights:	© 2024 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Borcherds
Date Added:	14 Jan 2026 17:35
Last Modified:	14 Jan 2026 17:35
URI:	https://irep.ntu.ac.uk/id/eprint/55042

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