Purification of bioactive compounds from rosemary by-products for innovative solutions in food industry

Ziani, I, Bouakline, H, Merzouki, M, Fauconnier, M, Sher, F ORCID: https://orcid.org/0000-0003-2890-5912, Bentouhami, NE, Asehraou, A and El Bachiri, A, 2025. Purification of bioactive compounds from rosemary by-products for innovative solutions in food industry. Industrial Crops and Products, 223: 120125. ISSN 0926-6690

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Abstract

Maximizing the value of agricultural by-products is critical for advancing sustainable development and minimizing waste. This study delves into the solid by-products of Rosmarinus tournefortii de Noé, focusing on their fractionation and purification to unveil valuable bioactive compounds. Using flash chromatography, nine distinct fractions (F1 to F9) were successfully isolated. Cutting-edge analytical techniques including FTIR, UHPLC-MS/MS, and 2D NMR (HSQC, HMBC and COSY) confirmed the presence of novel labdane diterpenoids (PF1), 24-nor-ursane triterpenoids (PF5), and ent-kaurane diterpenoids (PF6). Furthermore, HPLC-DAD profiling revealed rosmadial (79.43 % in F4) and luteolin (70.14 % in F7) as the dominant phenolic compounds. Notably, the crude extract demonstrated remarkable antioxidant activity, with an IC50 of 0.04±0.23 mg/mL. Fractions F7 and F8 also exhibited strong antioxidant potential, with IC50 values of 0.35±0.07 mg/mL and 0.36±0.02 mg/mL, respectively. Purified fractions PF7 and F8, enriched with luteolin and 3-hydroxyflavone, proved highly effective for pigmentation control, with PF7 showing an IC50 of 0.045±0.007 mg/mL. In addition, antimicrobial assays revealed that the 24-nor-ursane triterpenoid and fraction F6, containing ent-kaurane and carnosol, displayed potent inhibitory effects against Rhodotorula glutinis (24.1 mm). Molecular docking studies further highlighted PF5 as a potent inhibitor of alpha-amylase (-5.856 kcal/mol) and tyrosinase (-4.385 kcal/mol), while PF1 surpassed acarbose in alpha-glucosidase inhibition with a binding energy of −5.898 kcal/mol. Collectively, these findings highlight the immense potential of R. tournefortii by-products as a rich source of bioactive compounds for health and skincare, offering promising, sustainable solutions for the pharmaceutical, cosmetic and food preservation industries.

Item Type:	Journal article
Publication Title:	Industrial Crops and Products
Creators:	Ziani, I., Bouakline, H., Merzouki, M., Fauconnier, M., Sher, F., Bentouhami, N.E., Asehraou, A. and El Bachiri, A.
Publisher:	Elsevier BV
Date:	January 2025
Volume:	223
ISSN:	0926-6690
Identifiers:	Number Type 10.1016/j.indcrop.2024.120125 DOI S0926669024021022 Publisher Item Identifier 2556288 Other
Rights:	© 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Divisions:	Schools > School of Science and Technology
Record created by:	Melissa Cornwell
Date Added:	15 Jan 2026 10:13
Last Modified:	15 Jan 2026 10:15
URI:	https://irep.ntu.ac.uk/id/eprint/55049

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