Genotypic and phenotypic characteristics of Cronobacter species, with particular attention to the newly reclassified species C. helveticus, C. pulveris, and C. zurichensis [forthcoming]

Jackson, E, Sonbol, H, Masood, N and Forsythe, SJ ORCID: https://orcid.org/0000-0002-6709-0712, 2014. Genotypic and phenotypic characteristics of Cronobacter species, with particular attention to the newly reclassified species C. helveticus, C. pulveris, and C. zurichensis [forthcoming]. Food Microbiology. ISSN 0740-0020

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Abstract

In 2013, Enterobacter helveticus, E. pulveris and E. turicensis, were reclassified as Cronobacter helveticus, C. pulveris and C. zurichensis, respectively. Previously these species had been used as negative controls for some Cronobacter detection assays. This study examined cultural, biochemical and molecular Cronobacter detection and identification assays, with emphasis on the new species. Additionally, 32 Cronobacter genomes were examined for the presence of PCR target genes using the BLAST function of the online Cronobacter BIGSdb facility. The results of the cultural methods varied and no single medium was able to correctly detect all Cronobacter spp. Since the supporting databases have not been updated to include the Cronobacter genus, Enterobacter sakazakii was returned for four strains of the newly reclassified species with ID32E and none with API 20E. PCR probes targeting rpoB and ompA could not correctly identify the new Cronobacter spp., due to primer specificity or absent target genes. As neonates have been identified as a high-risk group for infection, international standards require the absence of all Cronobacter species in powdered infant formula. However, many conventional detection methods cannot correctly identify the newly recognized species. Conversely, DNA sequence-based methods can adapt to taxonomic revisions and will likely become more common.

Item Type:	Journal article
Publication Title:	Food Microbiology
Creators:	Jackson, E., Sonbol, H., Masood, N. and Forsythe, S.J.
Publisher:	Elsevier
Date:	2014
ISSN:	0740-0020
Identifiers:	Number Type 10.1016/j.fm.2014.06.013 DOI
Divisions:	Schools > School of Science and Technology
Record created by:	EPrints Services
Date Added:	09 Oct 2015 09:58
Last Modified:	09 Jun 2017 13:15
URI:	https://irep.ntu.ac.uk/id/eprint/5666

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