Bacterial cell wall constituents in the body fluids of patients in disease and in health

Cheetham, P, 1990. Bacterial cell wall constituents in the body fluids of patients in disease and in health. MPhil, Nottingham Trent University.

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Abstract

The development of a method for the detection and measurement of the amino sugar subunits of peptidoglycan in the body fluids of patients involved a number of stages. A number of derivatisation procedures were tested to find the most suitable. The gas chromatography conditions were investigated to obtain the optimum results. A combination of the most effective derivatisation reagent and chromatography conditions were used to examine pure chemicals, bacterial extracts and clinical specimens. The feasibility of using gas chromatography for detecting the amino sugars in body fluids was thus studied.

Results obtained with two of the derivatization reagents were promising when used with the pure chemicals. The examination of bacterial extracts demonstrated a lack of sensitivity of the methods used. The examination of clinical samples also illustrated this lack of sensitivity and the interference from other compounds. The examination of amino sugars in body fluids is not a feasible alternative to conventional culture and microscopy techniques.

Item Type:	Thesis
Creators:	Cheetham, P.
Date:	1990
ISBN:	9781369317060
Identifiers:	Number Type PQ10183542 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	01 Oct 2020 14:26
Last Modified:	20 Sep 2023 09:43
URI:	https://irep.ntu.ac.uk/id/eprint/41077

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